The RqlH PRTase domain is required for SOS induction in the absence of RqlI and RqlH-associated helicase activity.

On the left are schematic representations of the domains within the F11 RqlH protein and mutant variants that were expressed in MG1655 cells. Table summarizes the effects of each RqlH expression construct on bacterial growth (OD600), viability (CFU/ml), cell length, and SOS induction (determined using the reporter strain MG1655 attTn7::PsulA-GFP). Results are from 6 h shaking cultures in modified M9 media, and are normalized to control bacteria carrying the empty vector pBAD33. #; phenotype is rescued by co-expression of RqlI. *; phenotype is exacerbated by co-expression of RqlI. See S5 and S6 Figs for full data.