Detecting metastatic tumors after ¿-irradiation using longitudinal molecular imaging and gene expression profiling of metastatic tumor nodules

Metastatic tumors in C6-L (rat glioma cells ) xenografted mice were studied after local treatment with fractionated ¿-IR. To accurately detect the metastatic nodules after ¿-IR, we observed the effect of ¿-IR on distant metastatic tumor growth. Metastatic nodules after ¿-IR indicated extensive colonization of C6-L cells in the lungs within 6 weeks after ¿-IR. Identified and described the molecular events occurring after ¿-IR through gene expression profiling to elucidate genetic changes (differentially expressed genes between the ¿-IR primary tumors vs. non-¿-IR primary tumors and metastatic lung nodules vs. ¿-IR primary tumors).本研究针对接受分次γ射线（γ-IR）局部治疗的、移植有C6-L（大鼠胶质瘤细胞）的异种移植小鼠的转移性肿瘤开展研究。为精准检测γ射线照射后的转移性结节，本研究观察了γ射线照射对远处转移性肿瘤生长的影响。结果显示，γ射线照射后形成的转移性结节表明，在照射后6周内，C6-L细胞已在肺部发生广泛定植。本研究通过基因表达谱分析，鉴定并描述了γ射线照射后发生的分子事件，以阐明相关遗传变化——即γ射线照射原发肿瘤与非照射原发肿瘤之间、转移性肺结节与γ射线照射原发肿瘤之间的差异表达基因。