Agonist (carbachol) and antagonist (atropine) affinities expressed as pEC50 or pA2 respectively on the isolated mice ileum and distal colon of control mice and mice fed Curcuma extract over seven days.

aTissues from healthy mice fed standard diet over seven days.btissues from healthy mice fed Curcuma extract over seven days.cpEC50 = –log EC50. EC50 values are the means ± SE of at least four independent experiments and were calculated by a non linear regression curve-fitting computer program (47).dpA2 values ± SE were calculated from Schild plots [48] constrained to slope –1.0 [49]). pA2 is the positive value of the intercept of the line derived by plotting log (DR –1) vs log [antagonist]. The log (DR –1) was calculated from three different antagonist concentrations, and each concentration was tested from four to six times. Dose-ratio (DR) values represent the ratio of the potency of the agonist carbachol (EC50) in the presence of the antagonist and in its absence. Parallelism of concentration–response curves was checked by linear regression, and slopes were tested for significance (p