Identification of proteins bound with RPA194 at rRNA loci in primary versus metastatic breast cancer cells

The nucleolus, the site of ribosome biogenesis, serves as a central hub for sensing and responding to cellular stress and plays a crucial role in this process; furthermore, emerging evidence highlights the potential role of ribosome biogenesis in driving metastasis. A combined approach of chromatin immunoprecipitation (ChIP) with mass spectrometry identified unique binding partners of RPA194 (the major subunit of RNA polI) in metastatic versus non-metastatic breast cancer cells.