Culturing Patient-Derived Malignant Hematopoietic Stem Cells in Engineered and Fully Humanized 3D Niches

Data underlying the figures in the publication “Culturing patient-derived malignant hematopoietic stem cells in engineered and fully humanized 3D niches”, published in <em>PNAS,</em><em> <strong>2021</strong>, 118 (40) e2114227118.</em> <em>DOI: 10.1073/pnas.2114227118</em> Table of contents: <strong>1. AML data_Gene expression</strong>: qPCR data related to AML samples. <strong>2. AML data_Protein</strong>: Luminex data related to AML samples. <strong>3. AML data_Supernatant vs Niche</strong>: FACS data comparing AML cells in the supernatant and niche compartments. <strong>4. AML data_Supernatant</strong>: FACS data showing AML cells in the supernatant along the time. <strong>5. Chemotherapy_2D_FACS</strong>: FACS data showing leukemic cell viability in 2D. <strong>6. Chemotherapy_3D_FACS</strong>: FACS data showing leukemic cell viability in 3D (engineered niches). <strong>7. MPN data</strong>: FACS data related to MPN samples. <strong>8. SV-N_FACS</strong>: FACS data characterizing SV-N. <strong>9. SV-N_Gene expression</strong>: qPCR data characterizing SV-N. <strong>10. UCSD-AML1_Niche_FACS</strong>: FACS data showing UCSD-AML1 cells in the niche at final analysis. <strong>11. UCSD-AML1_Niche_Gene expression</strong>: qPCR data showing UCSD-AML1 cells in the niche at final analysis. <strong>12. UCSD-AML1_Supernatant_FACS</strong>: FACS data showing UCSD-AML1 cells in the supernatant along the time. <strong>13. UCSD-AML1_Supernatant_Gene expression</strong>: qPCR data related to UCSD-AML1 cells in the supernatant along the time. <strong>14. UCSD-AML1_Supernatant_Protein</strong>: Luminex data related to UCSD-AML1 cells in the supernatant along the time. <strong>15. Images engineered niches</strong>: Zip archive containing images of engineered niches.