MiRNA isoforms, tRNA-derived fragments, and rRNA-derived fragments have enrichments that differ characteristically among sub-cellular compartments and among cell lines (supplemental)

This supplemental BioProject for PRJNA816866 contains short RNA-seq of MDA-MB-468 cells treated with T4 Polynucleotide Kinase (T4 PNK). MDA-MB468 (ATCC #HTB-132) cells were grown according to standard cell growth conditions and treated with T4 PNK. RNA libraries were prepared for sequencing using standard Illumina TruSeq Small RNA protocols. The TruSeq Small RNA 3'-adapter is TGGAATTCTCGGGTGCCAAGG. Short RNA-seq was run on the Illumina NextSeq 500 sequencer.