B. pseudomallei 122018 and 266152 assays designed in this study.
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aUnderlined nucleotides indicate the position of the SNP in the TaqMan probe; lowercase nucleotides indicate a deliberately incorporated 5′ flap to enhance amplification efficiency [43].bBased on B. pseudomallei K96243 genome (GenBank Accession numbers BX571965 and BX571966 for chromosomes 1 and 2, respectively) [14].c‘Other species’ refers specifically to B. thailandensis, B. oklahomensis and B. thailandensis-like spp. B. mallei and other Burkholderia spp. (e.g. B. vietnamiensis, B. ubonensis etc.) do not amplify according to in silico and wet-bench analyses.
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2015-12-02



