Transcriptome profiles of differentiated wt1-SAM brown adipocytes treated with different sgRNA encoding plasmids and stimulated with isoproterenol

Cold and nutrient activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via uncoupled respiration and secretion of endocrine factors thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to inter- scapular brown adipose tissue (iBAT) function in high fat diet (HFD) and cold stressed mice. We focused on Gm15551 which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis and downregulated by β-adrenergic activation in mature adipocytes. Albeit we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551. polyA RNA profiles of brown adipocytes (wt1-SAM) differentiated for 7 days, reverse transfected with different sgRNAs at day 4 of differentiation and stimulated for 24 h with isoproterenol before harvesting were generated by deep sequencing in triplicate.