Data on the bioluminescence, cell viability, and biofilm formation of Photobacterium leiognathi strains exposed to ground microplasticss

ABSTRACT
This dataset provides comprehensive measurements of bioluminescence, cell viability, and biofilm formation in Photobacterium leiognathi strains (LB01 and LB09) exposed to varying concentrations of ground microplastics (GMPs). The study aims to investigate the effects of GMPs exposure on these critical bacterial functions, offering insights into the environmental impact of MPs on marine luminous bacteria.

DATA DESCRIPTION
The bioluminescence data, which expressed as specific bioluminescence (SBF), includes measurements recorded in Log relative light units (Log RLUs) using Glomax Discover (Promega Instrument). These measurements were taken at multiple time intervals, such as every 5 h for 3.1 days (75 h) or 24 h for 7 days, to capture the temporal changes in bioluminescence activity during and after (post-exposure experiment) exposure to GMPs (Tables S1-S4).
For cell viability, the data represented as optical density (ODs) at 560 nm. MTT assays were conducted to determine cell viability. The wells were added with MTT solution (5 mg/mL concentration), incubated for 8 h and dissolved with dimethyl sulfoxide. This analysis enabling an examination of the impact of GMPs on bacterial growth and survival (Tables S5-S6).
Biofilm formation data was obtained by quantifying the biofilm biomass using 1% crystal violet solution. This involved measuring the ODs at 570 nm to determine the amount of biofilm produced by Phb. leiognathi under each condition. The dataset includes biofilm measurements for both the control (unexposed to GMPs) and experimental groups, corresponding to the same GMP concentrations used in the bioluminescence and cell viability experiments (Tables S7-S8).

This dataset is valuable for researchers studying the environmental effects of microplastics on marine bacteria, particularly those interested in the functional responses of bioluminescent organisms to plastic pollution. The comprehensive data on bioluminescence, cell viability, and biofilm formation provides a robust foundation for understanding the ecophysiological effects of GMPs to Phb. leiognathi.