Diffraction images of crystals of the first and second spectrin repeats (mutant C420A/C435A) of human plectin (PDB code 2ODV): 2-wavelength SeMet MAD dataset

Diffraction images of SeMet labeled crystals of a fragment of human plectin that includes the first and second spectrin repeats (SR1-SR2) of the plakin domain. The two Cys in the wild type sequence were replaced by Ala.

This Se-Met MAD dataset was used for the de novo phasing of the pdb entry 2ODV (http://www.rcsb.org/pdb/explore/explore.do?structureId=2ODV).

Data was collected at the BM14 beamline of the European Synchrotron Radiation Facility (ESRF, Grenoble, France) using a Mar CCD detector. Data from the same crystal were collected at two wavelengths :

Remote wavelength (0.9185 Å): 180 images (1 degree oscillation per image).

Peak wavelength ( 0.9785 Å): 360 images (1 degree oscillation per image).