Association of hepatitis B virus genomes with active chromatin hubs challenges host replication fidelity, leading to DNA damage.

We have developed a modified iteration of a viral chromosome conformation capture (V3C-seq) assay to degrade non-cccDNA to show that the HBV cccDNA localizes spatially with cellular sites of DNA damage. Overall design: Identification of cellular interaction site of Hepatitis B Virus, and the localization of the resultant DNA damage response. Independent replicates of chromosome conformation capture (designated as V3C in the file name) and ChIP-seq experiments (the target epitope provided in the name) have been performed. For each time-point or treatment, at least two independent replicates of the indicated assays have been performed.