Human iPSC-derived monocytes and macrophages

Macrophages are pivotal effectors of host immunity and regulators of tissue homeostasis. Understanding of human macrophage biology has been hampered by the lack of reliable and scalable models for cellular and genetic studies. Human iPSC-derived monocytes and macrophages, as an unlimited source of subject genotype-specific cells, will undoubtedly play an important role in advancing our understanding of the role of macrophage biology and implication in human diseases. Here, we present RNA-Seq data of hiPSC-derived monocytes and GM-CSF or M-CSF macrophages generated by a fully optimized differentiation protocol as well as primary myeloid cells. Analysis of bulk RNA-Seq samples from hiPSC-derived monocytes and GM-CSF or M-CSF macrophages (n=5) as well as primary myeloid cells (monocytes, GM-CSF or M-CSF macrophages, n=6). One hiPSC-derived M-CSF macrophages sequencing library did not pass quality check.