Small RNA sequencing of brain-derived extracellular vesicles from post-mortem brain tissue of controls and Alzheimer's disease patients with different APOE genotypes

Using a recently optimized method for separation of brain-derived EVs (bdEVs) from post-mortem tissues, we obtained bdEVs of 23 AD patients with different APOE genotypes and 7 controls. RNA was extracted from brain homogenate, an intermediate ("10K") EV fraction, and more purified EVs and subjected to small RNA sequencing. Numerous RNA species varied with AD pathology and APOE genotypes. Dysregulation of some of these molecules has been implicated in amyloid processing, neurodegeneration and metabolic functions. Molecular differences of bdEVs may thus reflect or play a role in modulating AD progression. Total RNA was extracted from brain tissue (BH), 10K, and EVs of 23 AD patient with different APOE genotypes, as well as from 7 control brains.