Analysing environmentally induced genome-wide frameshift in S. cerevisiae using SLAM-Seq

In this study, we examine the impact of poor nutrient conditions on -1 ribosome frameshifting and its effect on mRNA degradation. To measure the RNA degradation, we utilized SLAM-seq (Herzog, et.al, 2017, PMID: 28945705) in S.cerevisiae in wildtype and NMD mutant (upf1Δ). Our goal with SLAM-seq is to quantify the fraction of the transcriptome that is directly degraded as a result of environmentally induced ribosome frameshifts through an NMD-dependent mechanism.本研究考察了营养匮乏条件对-1核糖体移码（-1 ribosome frameshifting）的影响，以及该移码事件对mRNA降解的调控作用。为定量检测RNA降解水平，我们在野生型及无义介导的mRNA降解（Nonsense-mediated mRNA decay, NMD）突变株upf1Δ的酿酒酵母（Saccharomyces cerevisiae，简称S. cerevisiae）中应用了SLAM-seq技术（Herzog等，2017，PMID: 28945705）。本研究借助SLAM-seq技术的核心目标是，定量分析因环境诱导的核糖体移码事件，并通过NMD依赖途径发生直接降解的转录组占比。