Plasmid pSTR2M1 with the SAT1 Flipper knockout cassette construction

The goal of the study was to obtain plasmid containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (STR2) and to introduce a cleavage site for restriction enzymes into them. The constructed cassettes will enable the removal of the tested genes from the C. albicans SC5314 genome using the SAT1-flipper method. The deletion of tested genes encoding cystathionine-γ- synthase STR2p enable the assessment of the impact of this enzyme on the phenotype of cells, on the virulence of the strain, and allows to recognize the in vivo function of the tested proteins in L-methionine biosynthesis. The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).