RFX3 loss disrupts non-coding RNA networks in iPSC-derived pancreatic progenitors

Project manager(s): Essam M. Abdelalim (Sidra Medicine, Qatar) Wild type iPSCs (WT-iPSCs) generated and fully characterized in our laboratory were used for RFX3 knockout iPSCs (RFX3 KO iPSCs) generation. WT-iPSCs and RFX3 KO iPSCs were differentiated into pancreatic progenitor cells to uncover RFX3’s role in regulating coding and non-coding RNA network at pancreatic progenitor stage as described in the article titled “RFX3 loss disrupts non-coding RNA networks in iPSC-derived pancreatic progenitors” by Aldous et al. The dataset represents small RNA-seq data generated from pancreatic progenitors (PPs) derived from WT-iPSCs and RFX3 KO iPSCs. The file name is: Sample name _overall sample number_read direction_001 where: - PPs-WT-Rep1: pancreatic progenitors (PPs) derived from WT-iPSCs, replicate 1. - PPs-WT-Rep2: pancreatic progenitors (PPs) derived from WT-iPSCs, replicate 2. - PPs-WT-Rep3: pancreatic progenitors (PPs) derived from WT-iPSCs, replicate 3. - PPs-KO1-Rep1: pancreatic progenitors (PPs) derived from RFX3 KO1 iPSCs, replicate 1. - PPs-KO1-Rep2: pancreatic progenitors (PPs) derived from RFX3 KO1 iPSCs, replicate 2. - PPs-KO1-Rep3: pancreatic progenitors (PPs) derived from RFX3 KO1 iPSCs, replicate 3. - PPs-KO2-Rep1: pancreatic progenitors (PPs) derived from RFX3 KO2 iPSCs, replicate 1. - PPs-KO2-Rep2: pancreatic progenitors (PPs) derived from RFX3 KO2 iPSCs, replicate 2. - PPs-KO2-Rep3: pancreatic progenitors (PPs) derived from RFX3 KO2 iPSCs, replicate 3. - Read direction: R1 (Forward).