Multi-dimensional transcriptome analysis of an intact hematopoietic organ for HSPC expansion [Bulk RNA-seq]
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https://www.ncbi.nlm.nih.gov/sra/SRP162761
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To characterize the distinct features of HSPCs and relevant niche cells, we performed RNA-seq with sorted zebrafish HSPCs and niche cells, based on fluorescent protein labeling from distinct regions at six relatively discrete stages. Overall design: We used double transgenic line Tg (kdrl:mCherry/CD41:GFP) and sorted HSCs, ECs and NCs from embryonic tails at 36 hpf, 52 hpf, 3 dpf and 4 dpf. And we sorted HSPCs and NCs from CD41: GFP kidney marrow at 3 mpf.
创建时间:
2021-04-29



