RNAseq (10x and SMARTseq) of the tergal gland of Dalotia coriaria

We deploy single-cell RNA sequencing (10x) and tissue specific ultra-low input RNAseq methods to delineate the molecular evolution of a rove beetle gland. We deconstruct the molecular assembly of new cellular functions comprising a biosynthetic organ novelty and uncover how gland function was pieced together from ancestral molecular source material through the assembly of two novel secretory cell types, each of which synthesize distinct compound classes.