Transcriptomic changes in paired eutopic and ectopic endometrium of ovarian endometriosis

The pathogenesis and mechanisms underlying endometriosis are still not fully understood. Emerging studies highlight that altered genes expressions may play a role in disease onset and progression. This research investigates critical genes gain further insight into the molecular basis of endometriosis. Through high-throughput sequencing, we performed paired expression analyses of mRNAs, microRNAs and lncRNAs in ectopic and paired eutopic endometrial tissues from five patients with ovarian endometriosis.Our findings shed new light on the regulatory networks involved, potentially guiding future strategies for replacement therapy in endometriosis. Overall design: Five pairs of ectopic and eutopic endometrium were sent for whole-transcriptome sequencing. In brief, samples of eutopic endometrium and ectopic endometrioma from the cyst walls were obtained immediately from 5 patients, ages 23 to 40 years old, who underwent hysteroscopy combined with laparoscopy for endometriosis, forming five pairs of self-control groups. All women had regular menstrual cycles and were not taking hormone therapy. According to the preoperative history, the menstrual phase was confirmed, and all samples were in the proliferative phase. The samples were sequenced using the Illumina HiSeq 2000/2500 platform, and 50-bp single-end reads were generated. Sequencing and data collection were conducted by Novogene Bioinformatics Technology Co., Ltd. (Beijing, China).