Differential transcriptional effects of mycobacterial nucleomodulins MgdE and MmpE in THP-1 macrophages

This study uses RNA-seq to quantitatively compare the gene expression profiles of THP-1 macrophages infected with the wild-type (WT), mgdE-deleted (?mgdE), and mmpE-deleted (?mmpE) strains of Mycobacterium bovis BCG, in order to investigate the roles of two conserved nucleomodulins, MgdE (Rv1075c) and MmpE (Rv2577), in host-pathogen interactions. Overall design: RNA-seq profiling was performed on THP-1 macrophages infected for 24 h with wild-type (WT), ?mgdE, and ?mmpE strains of M. bovis BCG. Each condition included three biological replicates, with the WT group serving as a shared control for both mutants