Supplementary information files for "Microfibre ingestion by the Asian Clam (Corbicula fluminea) is dependent on fibre type and biofilm development"

Supplementary files for article "Microfibre ingestion by the Asian Clam (Corbicula fluminea) is dependent on fibre type and biofilm development"<br><br>Fibrous microplastics represent an anthropogenic pollutant affecting aquatic systems globally. However, fibres formed from natural materials (e.g., cotton or wool) have only recently been recognised as potentially posing similar ecological threats as their synthetic counterparts. In this study we employed a laboratory-based aquarium experiment to examine the ingestion of preselected anthropogenic (polyester – microplastic) and ‘natural’ (cotton) microfibres by the Asian Clam (Corbicula fluminea). We considered how the ingestion, retention, and rejection of preselected microfibres (specific, distinctive colours), differed associated with fibre type (cotton vs polyester), biofilm development (control – no biofilm / uncultured, 1-week culturing and 4-week culturing) and time (1-48 hours). We found that the ingestion of microfibres was complex, dependent on the interaction of culturing and fibre type. Greater retention of synthetic microfibres was recorded compared to ‘natural’ microfibres as the duration of culturing increased. We also observed that ingestion of microfibres was immediate but that microfibres were rejected and visually observed in pseudofaeces. Our results suggest that the time microfibres spend within the environment, allowing biofilm to develop on their surface, influences the ingestion of microfibres and we call for further studies to consider this in the future.<br>© The Author(s) CC-BY-NC-ND 4.0