Recognition helix (RH) amino acid sequences and B2H activities of zinc finger arrays for endogenous zebrafish gene targets.

Each OPEN zinc finger array was assigned an OZ___ designation which permits their unique identification in the web-based Zinc Finger Database (ZiFDB) program [47]. Previously published zinc finger arrays targeted to the kdr gene (isolated by B1H selection) [27] are also shown. Each nine bp target site was named as follows: ��gene name or abbreviation��, ��exon number��, and ��L�� or ��R�� indicating left or right half-site. The amino acids selected in the three zinc finger recognition helices of each array are shown (residues are shown left to right in the order ?1, 1, 2, 3, 4, 5, 6 numbered relative to the helix start). B2H values that fall below the cut-off of three-fold activation in the B2H system are italicized. The names of zinc finger arrays tested as ZFNs in zebrafish are shown in bold italics. Abbreviations key: DT = dopamine transporter, HIF = hif1aa, Telo = telomerase, TfR2 = transferrin receptor 2, and Grck = gridlock.