RNA-Seq of injured mouse hearts depleted for myofibroblast Yap [I]

A myofibroblast specific tamoxifen inducible Cre was used to assess the contribution of Yap in the mouse heart after myocardial infarction We implemented a Cre-lox system whereby inducible Cre expressed under the myofibroblast specific Periostin promoter is crossed with Yap floxed animals, facilitating inducible depletion of Yap. Mice were siblings with floxed Yap alleles and either a copy of MerCreMer under the endogenous periostin promote or wildtype Periostin. Permenant ligation of the left anterior descending artery or sham surgeries were conducted. Mice were administered tamoxifen citrate diet ad libitum immediately after surgery to promote excession of myofibroblast yap and left ventricles were collected 4 days post injury.