Immunoreactivities of the tau exon-specific antibodies with six recombinant human tau isoforms.

(A) Schematic representation of six human tau isoforms. (B) SDS-PAGE of the purifications of six expressed tau isoforms in E. coli. The lysates of bacteria and the purified proteins were separated on a 12% gel and stained with 0.25% Coomassie brilliant blue. (C) Western blots of the prepared antibodies with six recombinant human tau proteins. A commercial tau mAb Anti-Tau1 is used as the control. The blots with various antibodies are indicated on the right. Different tau isoforms are shown on the top. The relative molecular weights are shown on the left.