Proteomics of Serum Samples for the Exploration of the Pathological Mechanism of Obstetric Antiphospholipid Syndrome

Obstetric antiphospholipid
syndrome (OAPS) is a multisystem disorder
characterized by thrombosis or recurrent fetal loss. In this study,
we aim to explore the pathological mechanism of OAPS. Herein, we carried
out data-independent acquisition (DIA) mass spectrometry quantitative
proteomic analysis of serum samples from OAPS patients and healthy
controls. A set of 93 differentially expressed proteins was identified,
including 75 upregulated and 18 downregulated proteins compared with
the levels in controls. Those proteins are enriched in KEGG pathways
related to autoimmune diseases, allergic diseases, and pathogen infection.
Interestingly, metabolic pathways such as fatty acid degradation and
type I diabetes were enriched, indicating that OAPS is metabolic disease
related. The significantly increased triglyceride also supported this
idea. The differentially expressed proteins insulin-like growth factor-binding
protein-1 (IGFBP-1), C-reactive protein (CRP), and ferritin light
chain (FTL) were validated by ELISA. Our study presented a deep serum
proteomics of OAPS and advanced our understanding of OAPS pathogenesis.