NEON Biorepository Mosquito Collection (DNA Extracts)

This collection contains genetic extracts derived from select mosquito vouchers (Diptera: Culicidae; NEON sample class: mos_barcoding_in.geneticSampleID). When adult mosquitoes are active, sampling occurs (via CDC light traps) every two weeks at core sites and every four weeks at gradient sites. A sampling bout consists of trapping at up to ten plots per site (pre-2018, approximately 40 hours per plot; 2018 onward, decrease to 24 hours per plot). Annually, up to 10 individuals of each taxon and sex (where available) per domain are pointed or pinned, receive morphology-based identification by a taxonomist, and have genetic sequences produced at the cytochrome oxidase I barcode region. Barcodes of cytochrome oxidase I are generated per specimen and are cross-posted on the Barcode of Life Data Portal (http://www.boldsystems.org/). Genetic extracts are stored dehydrated in 96-well plates from the Canadian Centre for DNA Barcoding are archived in the NEON Biorepository at -80 degrees Celsius. When eluted into 20 uL, concentrations of 5-30 ng/uL are expected. See related links below for protocols and NEON related data products. Information about the Canadian Centre for DNA Barcoding can be found at https://ccdb.ca/.