LTP of inhibition at PV interneuron output synapses requires developmental BMP signaling

This dataset represents the raw data that gave rise to the study by Vickers et al., 2020 in Scientific Reports (DOI: 10.1038/s41598-020-66862-5). Please refer to the original publication regarding experimental design and methodological details of data acquisition and analysis.  Below we supply information on the provided metadata files (which, in turn, refer to individual raw data files), and essential details on the specific data formats:

raw data is organized in the datasets related to Figs. 1, 2, 3, 4-E, 4F-H of the paper (Vickers et al., 2020);

the metadata for each individual dataset, listing individual data filenames from the respective dataset, are stored in separate “.csv” files, one per each dataset. Field separator: comma;

individual metadata files for each dataset are described in the master metadata file “metadata_master.csv”;

patch clamp recordings (Figs. 1-3, 4F-H) are provided as “.dat” files, unmodified from the original version created by the acquisition software PatchMaster (HEKA Elektronik, Germany). Besides original PatchMaster software, these files can be imported using one of the following methods: I) via Igor Pro extension bpc_ReadHeka.xop (for 32-bit Igor Pro versions 5.xx - 6.37) by Holger Taschenberger (https://www.wavemetrics.com/project/bpc_ReadHeka); II) via Python script by Luke Campagnola (https://github.com/campagnola/heka_reader); III) via Matlab script HEKA PatchMaster Importer by Christian Keine (https://github.com/ChristianKeine/HEKA_Patchmaster_Importer);

exact names of patch-clamp traces used for each Figure are provided in the corresponding “.csv” metadata files. Traces names are given in the format: ExperimentName_1_SeriesNumber_SweepNumber_ChannelNumber. When “*” is used, it means that every sweep of corresponding series was used for analysis (e.g. Nov0317_1_13_*_2  all sweeps from series number 13 were used). Description in the metadata files also specifies which series correspond to which experimental epoch (baseline or plasticity testing), and to pharmacological manipulations, if applicable;

widefield fluorescent images of coronal brain slices, cropped images of subregions in the primary auditory cortex used for quantification, and confocal Z-stacks zooming onto single nuclei of neurons stained for FISH experiments (Fig. 4A-E) were converted into composite TIFF format from the proprietary formats of Axioscan Z1 slide scanning microscope and LSM700 confocal microscope (Carl Zeiss). TIFF format is readable by FIJI/ImageJ (https://fiji.sc/ or https://imagej.net/Fiji/Downloads). Pixel resolution and voxel sizes are embedded in these TIFF files. Assignment of the color channels for each image is described in the metadata file.