Sequencing of HVVMRXALLeA0131P08 BAC

Tillers are vegetative branches that develop from axillary buds located in the leaf axils at the base of many grasses. Genetic manipulation of tillering is a major objective in breeding for improved cereal yields and competition with weeds. Despite t his, very little is known about the molecular geneti bases of tiller development in important Triticeae crops such as barley (Hordeum vulgare) and wheat (Triticum aestivum). Recessive mutations at the barley Uniculme4 (Cul4)locus cause reduced tillering, deregulation of the number of axillary buds in an axil, and alterations in leaf proximal-distal patterning. We isolated the Cul4 gene by positional cloning and showed it encodes a BTB-ankyrin protein closely related to Arabidopsis BLADE-ON-PETIOLE1 (BOP1) and BOP2. To verify gene content and identify the flanking genes within the corresponding barley genomic region, a sequenced Bacterial Artificial Chromosome (BAC) clone was identified as matching the co-segregating BTB-ankyrin gene and the ortholog of the proximal Brachypodium gene Bradi2g60705. Here we present the sequence of the identified BAC clone HVVMRXALLeA0131P08, which was sequenced using 454 technology.