Proteome mapping of human gingival tissue in health and disease

Gingival tissue proteome is at the forefront of the periodontal research, yet have been hampered by lack of sufficient tissue-extraction methods. Using an emerging technology for tissue homogenization, pressure cycling technology (PCT), we characterize the gingival tissue proteomic profiles of both healthy (n=5) and disease (n=5) sites from five systemically healthy individuals (51.6± 4.3 years) with generalized chronic periodontitis. In brief, the tissue was lysed and digested in PCT, the peptide was further subjected to an Orbitrap Fusion mass spectrometer. Label-free quantification (LFQ) was performed by Progenesis QI using Mascot and Scaffold for identifying and sorting of protein conflict, respectively.