The effects of different chemical forms of iron fertilizer on cadium accumulation in dwarf Polish wheat are reflected in the differentially expressed genes

Purpose: To understand the effects of two different chemical forms of iron fertilizer on cadmium accumulation Methods:Cultivation and treatment for three weeks of dwarf Polish wheat seedlings by hydroponics, in triplicate, qRT–PCR validation was performed using TaqMan and SYBR Green assays Results: Iron fertilizer can effectively reduce cadmium concentration in plants Conclusions: Our study represents the different chemical forms of iron fertilizer have different mitigation effects on cadmium. The transcriptome gata showed that iron fertilizer have changed the cadimium metabolism The groups treated with two fertilizers and CdCl2 solution before collected.The samples were collected after one week treatment. The roots samples were immediately frozen in liquid nitrogen and stored at -80°C.It’s three biological replicates per treatment for subsequent RNA extraction and analysis.