Expression data from isoalted adult cardiomyocytes w/o Isopretrenol stimulation.

Sympathetic activation of β-adrenergic receptors (β-AR) represent a hallmark in the development of heart failure (HF). Genome-wide analysis of CaMKII-mediated H3S28p in response to chronic β-AR stress by chromatin-immunoprecipitation followed by massive genomic sequencing led to the identification of CaMKII-dependent H3S28p target genes.40 % of differentially H3S28p-enriched genomic regions were associated with differential, mostly increased expression of the nearest genes, pointing to CaMKII-dependent H3S28p as an activating histone mark. To investigate whether Iso-induced H3S28p is associated with changes of gene expression, we performed microarray analyses (Affymetrix) to compare mRNA expression in Adult mouse ventricular myocytes (AMVMs) obtained from CaMKII WT and DKO mice after Iso stimulation.