Data for: A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription

Proper transcription orchestrated by RNA polymerase II (RNPII) is crucial for cellular development, which relies on the phosphorylation state of RNPII’s carboxyl-terminal domain (CTD). Sporangia, developed from mycelia, are essential for the destructive oomycetes Phytophthora, remarkable transcriptional changes are observed during the morphological transition. However, how these changes are rapidly triggered and their relationship with the versatile RNPII-CTD phosphorylation remain enigmatic. Herein, we found that Phytophthora capsici had undergone an elevation of Ser5-phosphorylation in its uncanonical heptapeptide repeats of RNPII-CTD during sporangia development, which subsequently changed the chromosomal occupation of RNPII and primarily activated transcription of certain genes. A cyclin-dependent kinase, PcCDK7, was highly induced and phosphorylated RNPII-CTD during this morphological transition. Mechanistically, a novel DCL1-dependent microRNA, pcamiR1, was found to be a feedback ..., , , # Data for: A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription

Access this dataset on Dryad, DOI:10.5061/dryad.k3j9kd5gk

The dataset is about the study of a microRNA’s biological function in *Phytophthora*, which is derived from genetic study and phenotype analysis. The dataset containing, in separate sheets, the underlying numerical data and statistical analysis for Figure panels 1F, 2B, 2C, 2G, 3C, 3D, 3E, 3G, 4B, 4C, 4E, 4H, 5C, 5D, 5F, 5H, 6G, 8A, 8B, 8C, 8D, 9A, 9B and 9F in the manuscript titled “A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription” (DOI: [https://doi.org/10.1371/journal.ppat.1012138](https://doi.org/10.1371/journal.ppat.1012138).

## Description of the data and file structure

* Fig. 1F/2G/3G/5F/5H/6G/9F: The relative levels of different proteins in different samples. Units: 1.
* Fig. 2B/3C/3D/4B...