SUMOylation Regulates miR-34b/c Targeted Gene Expression Program [miRNA-seq]

The microRNAs known as miR-34 family suppress the expression of a suit of proteins involved in oncogenesis and pluripotency, including c-Myc. Their expression is frequently down regulated in cancers; however the regulation of their expression is not well understood. Through genome-wide miRNA profiling and mechanistic analysis, we identified an important role of SUMOylation in miR-34b/c expression, regulating the expression of c-Myc and all other tested miR-34 targets. Overall design: We established HCT116 cell line that can be induced to express shRNA targeting the catalytic subunit of the SUMO E1 enzyme, SAE2 by adding doxocycline (DOX). To rule out non-specific shRNA effects, we established two separate cell lines using two independent shRNAs Sh1 and Sh2.