TNF response in promyelocytic and granulocytic forms of HL60/S4 cells. TNF response in promyelocytic and granulocytic forms of HL60/S4 cells

The human promyelocytic cell line HL60/S4 can be differentiated into a granulocytic form with the addition of all-trans retinoic acid for 4 days. We investigated the conserved and differentiated responses to TNF in the granulocytic and promyelocytic forms of HL60/S4 cells. Overall design: Promyeloytic and granulocytic HL60/S4 cells were treated with 16ng/ml TNF or equivalent volume of vehicle (water) in calcium supplemented growth media for two hours, then RNA was extracted with Trizol LS and the Qiagen Rneasy mini kit.