Supplemental Figure 4.
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<strong>Supplemental Figure 4. </strong>C57BL/6 mice treated with antibiotics for 6 days and then gavaged with PBS, ATCC25526 (ATCC) or UMB-MBP-01 (MD) <em>Bifidobacteria</em>. Mice then treated with tacrolimus (3 mg/kg/d s.c.) for the next two days. Tissues harvested 2 days after bacterial gavage and analyzed by flow cytometry. MLN stained for <strong>A</strong>) B220+ B cells, <strong>B</strong>) CD4+ T cells, <strong>C</strong>) CD8+ T cells, <strong>D</strong>) CD4+Foxp3+ Treg, <strong>E</strong>) CD11c+ DC, and <strong>F</strong>) F4/80+ MF. PLN stained for <strong>G</strong>) B220+ B cells, <strong>H</strong>) CD4+ T cells, <strong>I</strong>) CD8+ T cells, <strong>J</strong>) CD4+Foxp3+ Treg, <strong>K</strong>) CD11c+ DC, and <strong>L</strong>) F4/80+ MF. Frozen tissue sections analyzed by immunohistochemistry for <strong>M</strong>) CD11c+ DC and <strong>N</strong>) F4/80+ MF. <strong>O</strong>) MLN stained for laminin a4 and a5, with their ratio depicted. Treatment groups compared using one-way ANOVA. ** p value < 0.01. Data representative of 2 separate experiments, 3 mice per group, i.e. 6 total mice per condition over 2 experiments.
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figshare
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2022-12-07
