Next Generation Sequencing Analysis of miRNA profiling in fibroblast or myofibroblast. Next Generation Sequencing Analysis of miRNA profiling in fibroblast or myofibroblast

We reported the application of next generation sequencing technology for high-throughput profiling of miRNA expression in human primary lung fibroblast Cells with undifferentiated (fibroblast) or differentiated (myofibroblast) status. By comparation the expression aboundence of known miRNAs between undifferentiated and differentiated type human primary lung fibroblast cells, we found both upregulated and downregulated miRNAs in fibroblast cells during differentiation. This study provides a basic condition for further investigation of the roles of the regulated miRNAs during differentiation in fibroblast cells. Overall design: Examination of miRNA profiling in fibroblast and myofibroblast subtypes of human primary lung fibroblast Cells