Supplemental Material for Kweon et al., 2018

Figure S1 sraKO
mutant flies display normal morphology in axonal projections from
PDF-expressing circadian pacemaker neurons.

Figure S2 sraKO
mutation decreases PER and TIM levels in PDF-expressing circadian pacemaker
neurons. Adult fly brains were dissected at ZT23 in LD cycles.

Figure S3 A
transgenic reporter for sra promoter activity (sra-Gal4) is
expressed in various brain regions of adult fly.

Figure S4 sra depletion in PDF-expressing clock
neurons is sufficient to dampen rhythm amplitude in free-running circadian
locomotor behaviors.

Figure S5 Conditional
depletion of sra in PDF-expressing neurons causes poor rhythmicity in
free-running locomotor behaviors.

Figure S6 Genomic
deletion of each calcineurin subunit weakens rhythm amplitude in free-running
circadian locomotor behaviors.

Figure S7 Pharmacological
inhibition of calcineurin reduces the steady-state levels of PER and TIM, but
not CLK proteins, in Drosophila S2 cells.

Table S1 sra mutant flies display poor rhythmicity in free-running locomotor
behaviors in DD.

Table S2 sra depletion in PDF-expressing clock neurons is sufficient to dampen
rhythm amplitude in free-running circadian locomotor behaviors.

Table S3 Conditional
depletion of sra in PDF-expressing neurons dampens free-running
circadian locomotor rhythms.

Table S4 sra overexpression in circadian pacemaker neurons disrupts free-running
locomotor rhythms in DD.

Table S5 Genomic
deletion of each calcineurin subunit weakens rhythm amplitude in free-running
circadian locomotor behaviors.

Table S6 A
constitutively active form of Pp2B-14D disrupts free-running circadian
behaviors in a dose-dependent manner.

Table S7 sra genetically suppresses Pp2B-14DACT to sustain
circadian locomotor rhythms.

Table S8 Oral
administration of cyclosporine A (CsA) lengthens circadian periods in
free-running locomotor rhythms of wild-type flies.