Sequencing data of the insert of p2NIL-1877US/DS produced during the generation of the ∆<i>rv</i><i>1877</i> mutant

This is the second construct generated during the cloning process leading to the final construct used to delete rv1877 in M.tbHere, the US and DS regions have been cloned into the p2NIL vector, and they are sequenced to check for integrity. Since the insert is large, many sequencing reactions were performed, using different primers, named primer -1 to primer 9. Therefore alignments were performed with all data, to ensure coverage of the entire region.The last file, is the in silico designed construct, used as template for screening by restriction enzyme digestion (RED) and for the sequencing alignments