Compensatory growth partially mediates the effects of stressors associated with climate change on amphibian physiology

Human-induced climate change and urbanization are predicted to dramatically impact landscape hydrology, which can have devastating impacts on aquatic organisms. For amphibians that rely on aquatic environments to breed and develop, it is essential to understand how the larval environment impacts development, condition, and performance later in life. Two important predicted impacts of climate change and urbanization are reduced hydroperiod and increased larval density. Here we explored how larval density and hydroperiod affect development, morphology, physiology, and immunity at metamorphosis and 35 days post metamorphosis in the frog Rana pipiens. We found that high density had a large negative impact on development and morphology, which resulted in longer larval periods, reduced likelihood of metamorphosis, smaller size at metamorphosis, shorter femur-to-body length ratio, and reduced microbiome species evenness compared to animals that developed in low-density conditions. However, ani..., Mesocosm design
The experiment took place at the Pymatuning Laboratory of Ecology in Linesville, Pennsylvania, United States. Sixteen large cattle tanks (770L, Rubbermaid, grey plastic) were cleaned with bleach and allowed to dry for 24h, then filled to a depth of 41cm (600L) with well water 9 weeks prior to the start of the experiment. Lids for the mesocosms were constructed using 50% shade cloth and weighted tubing to ensure no insect predators or other amphibians could inhabit the mesocosms. Leaf litter was gathered from a nearby wooded area and dried, and 250g was added to each mesocosm 6 weeks prior to the start of the experiment. Pond water (1L per mesocosm) with concentrated zooplankton caught with a zooplankton net was added to each mesocosm 7 weeks prior to the start of the experiment. Each mesocosm was fitted with two temperature loggers (HOBO temperature pendants, Onset etc.) set to record the water temperature every 30 minutes, with one 5cm above the bottom on the tank, and ..., , # Compensatory growth partially mediates the effects of stressors associated with climate change on amphibian physiology

[https://doi.org/10.5061/dryad.0cfxpnw71](https://doi.org/10.5061/dryad.0cfxpnw71)

## Description of the data and file structure

#### Survival and larval period of the tadpoles across treatments

***larval period data file:*** the number of days that each animal spent inside each mesocosm, to analyse the length of the larval period and how it is affected by the different density and drying treatments. This dataframe also includes survival within the mesocosms and after they emerged from the mesocosm. And the dataset includes morphological measurements like leg length, femur length, mass and snout to venter length.  This dataframe contains all the metadata for all the individual animals within this study, therefore this represent the metadata included in many of the other dataframes uploaded in this study. Because it includes the morphological data collected through...,