High-resolution mapping of the period landscape reveals polymorphism in cell cycle frequency tuning

Biological oscillators adapt to environmental changes with widely tunable frequencies, a property theoretical studies attributed to positive feedbacks. However, no experiments have tested this theory. Here, we created synthetic cells to independently tune the frequency and feedback strength of a cell-cycle oscillator, enabling continuous mapping of period landscape in response to network perturbations. We found that although inhibiting positive feedback of cyclin-dependent kinase (Cdk1) reduces the tunability, the reduction is not as significant as theoretically predicted, and the Cdk1-counteracting phosphatase, PP2A, provides additional machinery to ensure frequency regulation. Additionally, cells exhibit polymorphic responses to PP2A inhibition, showing a monomodal distribution of oscillatory cells at low or high PP2A inhibition or a bimodal distribution at both low and high inhibitions. We explained the polymorphism by a model of two interlinked bistable switches of Cdk1 and PP2A whe...