Evolution of the carbohydrate 6-O sulfotransferase (C6OST) family in vertebrates and report of CHST16, a previously unrecognized C6OST gene lost from amniotes

Master_table_C6OST.xlsx: Complete table of all identified C6OST sequences. Includes sequence names, chromosomal locations, database identifiers and annotation notes of all C6OST sequences identified in this study. Also includes complete list of species, species abbreviations and genome assemblies used in the study. Sequence names include species abbreviations followed by chromosome/linkage group designations (if available) and gene symbols. Asterisks indicate incomplete/partial sequences. Paralogs (within-species duplicates) with uncertain phylogenetic relationships are designated as (1of2), (2of2) et c. Master_C6OST_all.rtf: All identified C6OST sequences in FASTA format, in the same order as in Master_table_C6OST.xlsx. Rich Test Format file marking exon junctions in alternating colors. Master_C6OST_all.fasta: All identified C6OST sequences in FASTA format, in the same order as in Master_table_C6OST.xlsx. FASTA format file for alignment/sequence viewing applications. Ident_C6OST_seq.txt: List of identical C6OST sequences in this dataset. 190121_C6OST_full_align.fasta: Alignment including the full repertoire of C6OST sequences (CHST1, CHST2, CHST3, CHST4, CHST5, CHST6, CHST7, CHST16 and related genes) in a smaller set of species. 190121_C6OST_full_IQ-TREE.tar.gz: Phylogenetic analysis (IQ-TREE output files) for the full repertoire of C6OST sequences in a smaller set of species. This analysis corresponds to Figures 1-5 in the publication. The file 190305_CHST7_align.fasta.treefile includes the phylogenetic tree in Newick format. The following files correspond to the alignments and phylogenetic analyses for each of the C6OST subfamilies with the full representation of species. These analyses correspond to Supplementary Figures S1-S8 in the publication. Within the IQ-TREE output files, the files ending on .treefile include the phylogenetic trees in Newick format. 190130_CHST1_align.fasta 190130_CHST1_IQ-TREE.tar.gz190130_CHST2_align.fasta 190130_CHST2_IQ-TREE.tar.gz 190130_CHST3_align.fasta 190130_CHST3_IQ-TREE.tar.gz190130_CHST4-5-6_align.fasta 190130_CHST4-5-6_IQ-TREE.tar.gz190130_CHST16_align.fasta 190130_CHST16_IQ-TREE.tar.gz190305_CHST7_align.fasta 190305_CHST7_IQ-TREE.tar.gz Conserved_synteny_data.xlsx: Chromosomal/conserved synteny data. Includes chromosomal locations and database identifiers of all C6OST-neighboring genes identified in the study. Also includes all identified synteny blocks in the human, chicken, Western clawed frog, spotted gar, zebrafish and medaka genomes.