Supporting Data for "Histone H3 Lysine 9 Di-methylation Mediates Transcription Regulation on Damaged Chromatin"

Supporting Data for the Thesis Entitled "Histone H3 Lysine 9 Di-methylation Mediates Transcription Regulation on Damaged Chromatin"Cells respond to DNA double-strand breaks (DSBs) by inducing transcriptional silencing in cis on the damaged chromatin, which is critical for efficient repair processes. Here we identify H3K9me2 as a novel mediator of transcription silencing on damaged chromatin and present PHF8 as a histone demethylase that promotes timely transcription resumption following DSB repair. This supporting data contains raw data in addition to processed data illustrated in the thesis. The raw data consists of quantification files, immunofluorescence images, western blot images, and RT-qPCR data and timelapse photos.

本篇论文支持数据集旨在辅助阐述题为《组蛋白H3赖氨酸9二甲基化介导损伤染色质上的转录调控》的研究。细胞在应对DNA双链断裂（DSBs）时，通过在受损染色质上的顺式转录沉默应答，此过程对于高效修复过程至关重要。本研究中，我们鉴定出H3K9me2作为损伤染色质上转录沉默的新介导因子，并提出PHF8作为一种组蛋白去甲基化酶，能够促进DSBs修复后转录的及时恢复。该支持数据集包含了论文中展示的原始数据及处理数据。原始数据包括定量文件、免疫荧光图像、蛋白质印迹图像、RT-qPCR数据和时间分辨图像。