miRNAs' functions in ADHD

In our previous study, we found that WBC miRNA may serve as ADHD prediction biomarkers. Therefore, we wonder whether these biomarkers miRNAs may regulate the neuron cells. We had Human Cortical Neuronal cells (HCN-2) transfected with miR-126-5p, miR-140-3p or Scrambled control mimics, followed by observing the differentiation of HCN-2 cells. We found that miR-126-5p and miR-140-3p promoted HCN-2 differentiation. To understand why these two miRNAs promoted HCN-2 differentiation. We collected total RNA samples of these three sets and examined gene expression with Human Clariom D microarray assay.