Timelapse videos of Fig.1D and 2A

FoxO3a nuclear-cytoplasmic pulsing dynamics in 184A1 mammary epithelial cell line following growth-factor treatment with varying ERK/AKT activation loads. Assay DescriptionThe nuclear-cytoplasmic pulsing behavior of a FoxO3a fluorescent reporter (FoxO3aN400-Venus) expressed in the 184A1 mammary epithelial cell line was assessed at the single-cell level using live imaging of cells that were untreated or treated with one of six growth factors in the absence or presence of an AKT or MEK inhibitor.Complete raw images can be found in the HMS OMERO link below. Refer to platemap.pdf for the dosing and types of ligands, and inhibitors at each row,column combination.