Comparative gene expression profiling of telomerase-reverse transcriptase (TERT)-immortalised BJ fibroblasts transduced with pBabe empty vector control or HRASG12V

We use RNA-seq to characterise the transcriptional programs of proliferating BJ-TERT fibroblasts versus those undergoing HRASV12-induced senescence (14 days) Overall design: BJ-TERT fibroblasts were transduced with pBabe-puro empty vector or pBabe-puro-HRASG12V to induce senescence and at 14 days post-transduction, total RNA was extracted for RNA-seq.