ChIP-Seq analysis of H-NS binding profiles in a Salmonella strain with a tetRA cassette insertion in Pathogenicity-Island-1 grown in the presence or in the absence of the tetRA inducer AHTc

Nucleoid structuring protein H-NS silences the expression of Salmonella Pathogenicity Island 1 (SPI-1) by binding to the promoter region of SPI-1's master regulator. The experiment aims at testing whether transcription of sequences more than one Kb upstream of the regulator gene causes H-NS:DNA complexes throughout SPI-1 (and elsewhere) to disassemble (as suggested by functional assays).