Identification of lncRNA in early stage breast cancer and their prognostic implications

Breast cancer is a common malignancy among women with highest incidence rate worldwide. Increasing evidences affirm lncRNA function in tumor development and progression. The aberrant and temporal expression of lncRNA may be a fundamental event for breast tumor initiation. In this study, RNA sequencing was employed to understand lncRNA expression signature in early stage breast cancer to identify lncRNA of functional importance in tumorigenesis. Infiltrating ductal carcinoma (IDC) and Ductal carcinoma in situ along with adjacent/marginal tissues of respective IDC tissues as paired normal and tissues obtained from patients undergoing surgery for breast conditions other than malignancy were used as apparent normal samples. Tumor tissues samples were histopathologically confirmed of containing more than 70% of tumor cells. Total RNA isolated from these tissues were rRNA depleted and stranded library was synthesized.