Effects of plantain on transcriptional profile of liver in obese rats

We explored the mechanisms underlying the anti-obesity effect of plantain. Plantain flour was used to feed obese rats fed a high-fat diet. Six groups were set, viz., the normal control (NC), obese control (OC), low-dose (LD), middle-dose (MD), high-dose (HD), and positive control (PC). The differentially expressed genes in the liver, as well as the lipid and glucose metabolic pathways were investigated. Transcriptome analyses showed that the expression of the PPARα, CPT1A, AMPK, and PGC1α genes was upregulated in the plantain intervention groups. Conclusion: Some key genes in fatty acid and sugar metabolic pathways were regulated by the plantain diet, which probably led to the observed fat and weight loss effects.本研究探究了车前草抗肥胖效应的潜在作用机制。研究采用车前草粉饲喂高脂膳食造模的肥胖大鼠，设置6个实验组，分别为正常对照组（normal control, NC）、肥胖模型对照组（obese control, OC）、低剂量组（low-dose, LD）、中剂量组（middle-dose, MD）、高剂量组（high-dose, HD）及阳性对照组（positive control, PC）。本研究对大鼠肝脏组织中的差异表达基因以及脂类与葡萄糖代谢通路进行了分析。转录组分析结果显示，车前草干预组中PPARα、CPT1A、AMPK及PGC1α基因的表达水平显著上调。研究结论表明，车前草膳食可调控脂肪酸与糖代谢通路中的关键基因，这可能是其实现观测到的减脂与减重效应的潜在机制。