The CRISPR-Cas double nickase type
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
The invention provides for delivery， engineering and optimization of systems， methods， and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems， some of which encode one or more components of a CRISPR complex， as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity. The invention provides for delivery， engineering and optimization of systems， methods， and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems， some of which encode one or more components of a CRISPR complex， as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
