CIL:13911, Homo sapiens, permanent cell line cell. In Cell Image Library
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https://library.ucsd.edu/dc/object/bb6696114m
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Human colon carcinoma HCT 116 cells were transfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and enucleated to obtain cytoplasts. The cytoplasts were treated with vehicle (control for Resveratrol and Spermidine experiments). Nuclei were counterstained by Hoechst. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 8A, left panel, in Morselli et al. J Cell Biol 192: 615-629
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UC San Diego Library Digital Collections
创建时间:
2021-04-15



